Fig. 2

CCR5 expression in blood and cerebrospinal fluid Th lymphocytes from a TBE patient. The representative FACS plots demonstrating the gating strategy and the measured CCR5 (CD195) expression in CD3+CD4+CD45RO+ lymphocyte population in blood (peripheral blood mononuclear cells—PBMC, upper panel) and in cerebrospinal fluid (csf, lower panel) obtained on admission to hospital from a TBEV-infected patient. Left: T lymphocytes were gated based on CD3 and side scatter dot plot (gate R1). Middle: CD4+CD45RO+ T lymphocytes were gated in selected R1 population based on CD4 and CD45RO dot plot (gate R3). Isotype controls from the same blood and csf samples were used to define the CD45RO+ population. Gate R2 contains CD4+CD45-negative T lymphocytes. Right: a histogram of CD195 expression intensity (dark gray) measured on gate R3 (light gray—isotype control). The tendency for a preferential migration of T lymphocytes to csf (left), for the enrichment of the T lymphocyte population that has migrated into csf in CD4+CD45RO+ lymphocytes (center) and for the increased CCR5 expression in the csf CD4+CD45RO+ cells (right) is visible