Fig. 2From: Sustained TNF production by central nervous system infiltrating macrophages promotes progressive autoimmune encephalomyelitisTNF secretion is sustained in infiltrating macrophages during progressive EAE. TNF production by macrophages and microglia determined by intracellular staining during a acute (d19 p.i.) and b chronic (d30 p.i.) EAE in WT (black line) or GFAP∆R1γ (red line) mice. Numbers in histograms depict the percentage of TNF producing WT cells (black) or GFAP∆R1γ cells (red). Dotted line represents isotype control. Bar graphs show mean fluorescence intensity (MFI) of TNF staining in macrophages and microglia from WT and GFAP∆R1γ mice during acute and chronic disease. Data represent the mean values and ± SEM of three independent experiments with six mice per group per experiment. P values determined by Student’s t testBack to article page