Fig. 1

Stability and localisation of intravitreally injected NR58-3.14.3. a Levels of fluorescence readily increased in retinas injected with fluorescein-conjugated NR58-3.14.3 after only 20 min and remained highly elevated after 3 h. Fluorescence was significantly lower at 7 days post-injection (P < 0.05), but was still far higher than fluorescein-only- and non-injected controls (P < 0.05). b NR58-3.14.3-fluorescein (green, arrow) was present throughout the retina at 20 min post-injection. c Fluorescence remained consistent in retinas after 3 h, though was more concentrated within the GCL, NFL, INL, and RPE (arrows). d, e After 24 h and 7 days post-injection, fluorescence for NR58-3.14.3-fluorescein was markedly reduced but still readily apparent around the NFL, retinal vasculature, and RPE (arrows). f Retinas injected with fluorescein-only showed only very faint background fluorescence in comparison to NR58-3.14.3-fluorescein-injected animals (e). GCL ganglion cell layer, INL inner nuclear layer, NFL nerve fibre layer, ONL outer nuclear layer, RPE retinal pigment epithelium. Scale bars represent 100 μm. NR58-3.14.3-fluorescein n = 3, fluorescein-only n = 3, non-injected n = 3