Fig. 6

In vivo effects of PDTC on HO-1 expression, copper content and lipid peroxidation. a Representative immunostaining images of brain sections from WT mice treated with PDTC for 9 months with or without PDTC at a dose of 20 mg/kg/day. HO-1, GFAP, and nuclear DAPI were imaged from the hippocampal dentate gyrus. b The percentage of glial cells expressing HO-1 in the hippocampal dentate gyrus was calculated from five WT mice receiving PDTC for 9 months at a dose of 20 mg/kg/day and five vehicle-treated animals. Data are shown as percentage of HO-1 positive cells ± SD. c Cerebellar copper concentrations were measured from WT mice treated with PDTC for 2 weeks at a dose of 75 mg/kg/day by ICP-MS and are shown normalized to mass of the tissue ± SD. d Lipid peroxidation in hippocampi of WT mice treated for 9 months with or without PDTC at a dose of 20 mg/kg/day and in e WT and Nrf2^−/− hippocampi were measured by the FOX assay. Data are expressed as μM hydroperoxides per milligram protein ± SD. *p < 0.05; **p < 0.01