Fig. 6

Celastrol suppressed retinal leukostasis and retinal expression of ICAM-1 and VCAM-1 in light-exposed mice. Dark-adapted BALB/c mice were exposed to bright light at 10,000 lx for 2 h after pretreatment with either DMSO or celastrol at 5 mg/kg bw. a Fluorescein-conjugated ConA labeling for adherent leukocytes in retinal vasculature was examined in mice without bright light exposure (no light) and 3 h after light exposure. Retinal flatmounts were made and observed under fluorescent microscope. b The number of loci with adherent leukocytes per field in each retina was counted (9–14 fields per retina, n = 4–6 retinas per group). Scale bar: 100 μm. c Retinas were collected 6 h and 1 day after light exposure and subjected to real-time PCR analyses for expression of ICAM-1, VCAM-1, and VEGF, respectively. Relative fold change against that from the mice without bright light exposure (no light) was presented. Data were expressed as mean ± S.E.M. (n = 4–6 per group). *Compared to that from no light, p < 0.05; ^# compared to that from DMSO, p < 0.05