Fig. 7

Celastrol suppressed microglial activation and gliosis in the retinas of bright light-exposed BALB/c mice. Dark-adapted BALB/c mice were exposed to bright light at 10,000 lx for 2 h after pretreatment with either DMSO or celastrol at 5 mg/kg bw. Cryosections made from eye cups collected 3 days after light exposure were subjected to IHC examination for the expression of Iba-1 (in red) (n = 3–5 per group) (a). Cryosections made from eye cups collected 7 days after light exposure were subjected to IHC examination of GFAP (in red) (n = 3–5 per group) (b) and vimentin (in red) (c) (n = 3–5 per group). DAPI counterstaining (in blue) was performed to visualize retinal gross morphology. ONL outer nuclear layer, INL inner nuclear layer. Scale bar: 50 μm