Table 1 Primer sequences
Gene | Forward primer | Reverse primer |
|---|---|---|
Mouse IL1β | TGCCACCTTTTGACAGTGATG | AAGGTCCACGGGAAAGACAC |
Mouse Ccl2 | AGCTGTAGTTTTTGTCACCAAGC | GTGCTGAAGACCTTAGGGCA |
Mouse COX2 | CCGTACACATCATTTGAAGAACTTA | CTACCATGGTCTCCCCAAAGAT |
Mouse TNFα | ACGTCGTAGCAAACCACCAA | GCAGCCTTGTCCCTTGAAGA |
Mouse ICAM-1 | TCCGGACTTTCGATCTTCCAGCTAC | CCAGGTATATCCGAGCTTCAGAGGC |
Mouse VCAM-1 | AAGAAAGGGAGACTGTCAAAGAACT | AACTTCATTATCTAACTTCCTGCCC |
Mouse VEGF | GTACTTGCAGATGTGACAAGCCA | GGTGACATGGTTAATCGGTCTTT |
Mouse GAPDH | CCGGTGCTGAGTATGTCGT | CCTTTTGGCTCCACCCTTC |
Human IL1β | TTATTACAGTGGCAATGAGGATGAC | GGAAGGAGCACTTCATCTGTTTAG |
Human Ccl2 | CTCATAGCAGCCACCTTCATTC | CTCTGCACTGAGATCTTCCTATTG |
Human COX2 | GATTTGACCAGTATAAGTGCGATTG | GTTTGGAGTGGGTTTCAGAAATAAT |
Human TNFα | CCTCTCTCTAATCAGCCCTCTG | CTACAACATGGGCTACAGGCTT |
Human ICAM-1 | AAGATAGCCAACCAATGTGCTAT | AAGATAGCCAACCAATGTGCTAT |
Human GAPDH | ACTCTGGTAAAGTGGATATTGTTGC | GGAATCATATTGGAACATGTAAACC |