Fig. 4

p-JNK effects on α-SNC secretion do not depend on the transcriptional activity of cJUN. PC12 cells expressing α-SNC/p25α were differentiated with NGF and transduced with control vector or a dominant-negative cJUN-Δ169-FLAG construct in doses of viral supernatant of 25 or 50 μl before transgene (α-SNC/p25α) induction with doxycycline. a Four days post-infection cells were prepared for immunofluorescence with anti-FLAG antibodies as indicated. Alexa647-conjugated phalloidin (F-actin) was used as counter stain. Bar, 10 μm. b Cell lysates were prepared for western blotting with anti-FLAG mAb for the detection of cJUN-Δ169-FLAG. Arrow indicates 25 kDa weight marker. The WB is the representative of two independent experiments. c Conditioned medium from transduced cells as above was analyzed for α-SNC by western blotting. All lanes were derived from the same membrane. d Normalized mean and SEM integrated optical density of α-SNC western bands above for four independent experiments