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Fig. 8 | Journal of Neuroinflammation

Fig. 8

From: Reciprocal signals between microglia and neurons regulate α-synuclein secretion by exophagy through a neuronal cJUN-N-terminal kinase-signaling axis

Fig. 8

TNFα-induced α-SNC secretion from neurons requires JNK2 or JNK3, and non-inflammatory cytokines can increase α-SNC secretion. a PC12 cells expressing α-SNC and JNK1, JNK2, or scrambled control (Scr) shRNA were stimulated with TNFα (10 or 25 ng/ml) overnight. Whole cell lysate and conditioned media (TCA) were collected and analyzed by WB. Showing representative images from three independent experiments. b Differentiated SH-SY5Y cells expressing α-SNCA30P and JNK1, JNK2, JNK3, or scrambled shRNA were stimulated with TNFα (2 or 5 ng/ml) overnight. Whole cell lysates and conditioned media (TCA) were collected and analyzed by WB. The blot shown is representative of three independent experiments. c Quantification of secreted α-SNC (TCA) in a. Data are presented as mean fold increase + SEM (N = 3) relative to control “Scr” shRNA (*p < 0.05 and **p < 0.01 compared to Scr shRNA in “Control.” p values are based on comparison with Scr shRNA within each group, i.e., 10 or 25 ng/ml). d Quantification of secreted α-SNC (TCA) in b. Data are presented as mean fold increase + SEM (N = 3) relative to control “Scr” shRNA (**p < 0.01 compared to “Control”). e PC12 cells expressing α-SNCA30P were stimulated overnight with 100 ng/ml LPS or 10 ng/ml IL34, TGFβ1, or IFNβ1. Conditioned media (TCA) were collected and analyzed by WB. Showing representative image from three independent experiments. f Quantification of monomeric α-SNC in e. Data are presented as mean fold increase ± SEM (N = 3) relative to “Control” (**p < 0.01 and ***p < 0.001 compared to “Control”)

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