Fig. 1

Inhibition of the Nogo/NgR pathway reduced Aβ plaque deposition in APP/PS1 mice. a, b Mice brain sections from APP/PS1 mice were stained with thioflavin S and calculated as Aβ-positive area fraction in the hippocampal and cortical regions at 8 months of age. Bar = 100 μM. Values were reported as mean ± SD, as a percentage of values determined in the vehicle group (control, 100 %). The Aβ plaque in the brain was estimated after immunohistochemistry with Aβ antibody (6E10), and the position area fraction of the hippocampal and cortical regions of brain tissues were calculated (c, d). Bar = 100 μM. Values were reported as mean ± SD, as a percentage of values determined in the vehicle group (control, 100 %). e–h The cortex or hippocampus of APP/PS1 mice was homogenized and separated into TBS-, TBST-, and guanidine-soluble fractions. The proportions of Aβ42 (e, f) and Aβ40 (g, h) were measured using ELISA. e, g The cortex of APP/PS1 mice; f, h The hippocampus of APP/PS1 mice. Values were reported as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001, when compared with the vehicle group, n = 3–6