Fig. 8

Increasing of M1 inflammatory gene and decreasing of M2 inflammatory gene in Nogo/NgR-activated BV-2 microglia. Before added to the protein-coated wells, BV-2 microglia was transfected with control or NgR siRNA to suppress the expression of NgR. The cells were then stimulated by exposed to PBS or Nogo-P4 for 6 h. The levels of M1 (a–f) and M2 (g–l) inflammatory gene transcripts in BV-2 microglia were examined by quantitative RT-PCR. a iNOS. b IL-1β. c TNF-α. d IL-6. e COX-2. f CCL2. g Arg1. h Fizz1. i Ym1. j IL-4. k cd206. l IL-10. Values were reported as mean ± SD, as a percentage of values determined in PBS group (control, 100 %). *p < 0.05, **p < 0.01, ***p < 0.01, n = 4