Fig. 5

Brain PCs can generate microglia following ischemia. Some of the clustered MACS-sorted PDGFRβ⁺ iPCs (a) expressed Iba1 (b) (Iba1 (b green), DAPI (b blue)), but not CD68 (c) (CD68 (c green), DAPI (c blue)). The cell clusters differentiated into Iba1⁺ and CD11b⁺ microglia (Iba1 (d, e green), CD11b (d, f red), DAPI (d–f blue)). The differentiated Iba1⁺ ameboid-like microglia, but not the ramified-like microglia, expressed the MHC class 1 protein (arrow and arrowhead, respectively) (Iba1 (g, h red), MHC class I (g, i green), DAPI (g–j blue)). Electron microscopy revealed that differentiated cells have microglial traits and show increased electron density at the cytoplasmic periphery (k, arrow). Iba1⁺ ameboid-like microglia were able to phagocytose latex beads (Iba1 (l red), latex beads (l green), DAPI (l blue)). Scale bars = 50 μm (b, c), 20 μm (d, g), 1 μm (k), and 10 μm (l) Iba1 ionized calcium binding adaptor molecule 1, MACS magnetic cell sorting, MHC major histocompatibility complex, PDGFRβ platelet-derived growth factor receptor-β