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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Brain pericytes serve as microglia-generating multipotent vascular stem cells following ischemic stroke

Fig. 6

Oxygen/glucose deprivation induces brain PCs to acquire the traits of stem cells and microglia. Normal brain pericytes (PC-N) (a) were subjected to OGD (b). Immunohistochemistry showed that PC-N were positive for various pericytic markers, including PDGFRβ (PDGFRβ (c green), DAPI (c blue)), NG2 (NG2 (d green), DAPI (d blue)), and αSMA (αSMA (e green), DAPI (e blue)). PC-N expression of these pericytic markers was confirmed by RT-PCR (f). Following OGD treatment, PC-OGD showed a gradual decrease in pericytic/mesenchymal marker gene expression, including for NG2, PDGFRβ, and αSMA. In contrast, expression of the stem/epithelial cell marker Sox2 was upregulated (g). PC-OGD that formed cell clusters (h, i) showed strong expression of the stem cell markers Sox2 and nestin compared with that in the control cells (j). In addition, PC-OGD-associated cell clusters expressed Iba1 and CD11b (k). Scale bars = 50 μm (a, c, d, e, i) PC-N PCs cultured under normal/normoxia condition, PC-OGD PCs cultured under oxygen/glucose deprivation

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