Fig. 9

Delayed TO901317 treatment attenuated injury volume, hemispheric enlargement, and neuronal death after ICH. a Representative cresyl violet-stained brain sections of a vehicle-treated and a delayed 30 mg/kg TO901317-treated mouse at 4 days post-ICH. Analysis of lesion volumes demonstrates that treatment with 30 mg/kg TO901317 at 3 h post-ICH significantly reduced hemorrhagic injury volume and hemispheric enlargement at 4 days. The scale bar is 2 mm. b Representative FJB-stained sections of a sham-injured, a vehicle-treated, and a delayed 30 mg/kg TO901317-treated mouse at 4 days post-ICH. The inset is a representative FJB-positive cell at higher magnification. Quantification analysis shows that treatment with 30 mg/kg TO901317 at 3 h post-ICH significantly reduced the number of degenerating neurons at 4 days post-ICH. The scale bar is 100 μm. Values are presented as means ± SEM; ^## P < 0.01 and ^### P < 0.001 versus vehicle group (n = 7 mice/group, Student’s t test)