Fig. 3

Pronounced microglia activation in the septum with little or no apparent neurodegeneration. Dual labeling of microglia with IBA1 immunoreactivity and FJc to detect neurodegeneration is shown in the dorsal and more lateral aspects of the septum. The three left-hand panels show the DAB-labeled microglia with incandescent illumination while the three center, and three most right-hand panels are fluorescent images obtained using a TRITC filter (for FJc detection of neurodegeneration). The top left and center panels from a METH-treated animal (3 days post dosing) show that there is pronounced microglia activation with minimal evidence of neurodegeneration. The most intensely labeled structures in these dorsal lateral regions, indicated by purple arrows, are vasculature-related. The top right-hand panel shows the FJc-labeled degenerating puncta in the caudate putamen at high magnification of the same animal. The remaining six panels are from two different METH-treated animals that further show there is little or no FJc evidence of neurodegeneration in many of the dorsal septal regions with activated microglia. The purple arrows in two of the bottom panels outline a large vessel that is present. The blue arrows show where the region of high magnification resides in the low magnification panels