Fig. 9

Roles of K⁺ and CRAC channels in myelin phagocytosis and ROS production. Microglia were stimulated for 24 h (as in Fig. 8), and then a channel blocker was added with or without myelin for a further 6 h. The panels show single stimuli, as well as M2a→M1 (left), M2c→M1 (middle), and both M1→M2a and M1→M2c (right). a KCa3.1 inhibition using 1 μM TRAM-34. b Kv1.3 inhibition using 5 nM Agitoxin-2 (AgTx-2). c Kir2.1 inhibition using 20 μM ML133. d CRAC inhibition using 10 μM BTP2. Graphical data are expressed as mean ± SEM (n = 6 individual cultures) and were analyzed by a two-way ANOVA with Bonferroni’s post hoc test. The dashed lines indicate levels in unstimulated microglia with myelin. Comparisons are as follows: asterisk differences from control microglia, dagger sign CTL versus activation states in the presence of a channel inhibitor, number sign effect of a second stimulus on activated microglia, double dagger sign effect of a channel inhibitor within treatment group. One symbol p < 0.05, two symbols p < 0.01, three symbols p < 0.001