Fig. 4

T cell differentiation. a Lymph nodes cells were incubated in vitro for 6 h without activation and thereafter lysed for analysis by western blot as described in the “Methods” section. Representative blot for each protein studied. Band intensity was quantified and normalized to the intensity of β-actin. b Bar graphs showing the average relative value + SD of normalized levels of protein in lymph node cell lysates (n = 3–5 samples/group). c Bar graphs showing the average + SD titer of anti-α-syn antibodies in serum (μg/μL). All samples were done in duplicates (n = 3–5/group). One-way ANOVA followed by Tukey HSD. Asterisk means different from naive; A means different from adjuvant; not equal to symbol means different from all; double dagger symbol means different from the other α-syn variant at a different dose; plus-minus sign means different from the same α-syn variant at different dose. p < 0.05