Fig. 6

Expression of CCR6 and CD103. Cells were gated as before for CD3+CD4+Foxp3- (Th) and CD3+CD4+Foxp3+ (Treg) and thereafter for dopamine receptor expression: D2 (DR-D2+), D3 (DR-D3+) or double-negative (DR-D3-D2-). The expression of CCR6 and CD103 was determined in each of these populations. a Representative dot plot showing CCR6 and CD103 co-expression in Treg cells un-gated for DR. b–g Bar graphs representing the average percentage + SD of cells expressing CCR6 and CD103 in the total Treg (b) and Th (c) cell populations and in cells gated for DR-D2 (d, e) and DR-D3 (f, g). h Bar graph showing the average percentage + SD of CCR6+ and CD103+ cells in Treg cells negative for DR expression. One-way ANOVA followed by Fisher’s LSD. Asterisk means different from naive; A means different from adjuvant; open circle means different from the other α-syn variant independent of dose; not equal to symbol means different from all; plus-minus sign means different from the same α-syn variant at different dose; dollar sign means different from the other α-syn variant at the same dose. p < 0.05. N = 8–10, divided into three to five independent experiments