Fig. 8

Percentage of microglia expressing diverse activation markers. a Representative dot plots showing the gating strategy for live CD11b+ cells. Bar graphs representing the average percentage + SD of microglia expressing activation markers related to tolerance (b) or adaptive immunity (c). Tables showing the average percentage of co-expression between pairs of activation markers at low dose (d, f) and high dose (e, g), as well as adjuvant (h) and naive (i). Markers co-expressed in less than 30 % of the population are in blue (considered to have an independent expression), and those co-expressed in 70 % or more are in orange (considered to have a correlated expression). One-way ANOVA followed by Tukey HSD. Asterisk means different from naive; A means different from adjuvant; dagger symbol means different from naive and adjuvant; open circle means different from the other α-syn variant independent of dose; not equal to symbol means different from all; plus-minus sign means different from the same α-syn variant at different dose; dollar sign means different from the other α-syn variant at the same dose; double dagger symbol means different from the other α-syn variant at a different dose. p < 0.05. n = 8–10, divided into three independent experiments