Fig. 5

The CNS milieu of CXCR3^−/− mice promotes the proliferation of Th17 cells. Adoptive-transfer EAE was performed as described in Fig. 4. Recipient mice were injected with BrdU on day 11 post-immunization. At 24-h post-injection, the spinal cord was collected and CNS-infiltrating cells were isolated. The cells were immunostained with anti-CD4-APC-Cy7 followed by cell cycle analysis by quantification of cell-incorporated BrdU and total DNA content (7-AAD). a Representative flow cytometry plots for cell cycle analysis. b The percentage of CD4⁺ T cells in the apoptotic phase, G0/G1 phase, S phase, and G2/M phase of the cell cycle among the four groups. c The number of IL-17⁺ cells in CD4⁺BrdU⁺ (S phase) are shown for the four groups. Each group has 13 mice (n = 13). Each mouse is represented by a symbol. Data shown are from two independent experiments. **P < 0.01; ***P < 0.001