Fig. 7

IL-23 and CCL20 production are significantly increased in glial cells derived from the cortex of neonatal CXCR3^−/− mouse brains compared with those from WT mice. The brain cortex was collected from neonatal mice (day 1), and cells were disaggregated. Disaggregated cells were cultured in glial cell enrichment medium for 12 days followed by the separation of astrocytes (a) from microglia (b), as described in the “Methods” section. Astrocytes and microglia were stimulated with LPS for 24 h, and IL-23p19 and CCL20 levels in collected culture supernatants were measured by ELISA. Data represent means ± SEM from eight independent experiments with three neonatal mice for each experiment. *P < 0.05; **P < 0.01