Fig. 8

CXCR3^−/− mice show elevated numbers of glial cells and increased leukocyte infiltration and demyelination in the spinal cord during EAE. EAE was induced in WT and CXCR3^−/− mice as described in Fig. 1. At 15-days post-immunization, lumbar (a, b) and thoracic (c, d) spinal cords were collected and subjected to immunofluorescence staining (a, c) and H&E as well as LFB staining (b, d). Immunofluorescence staining for Iba1 (microglial cells) and GFAP (astrocytes) were performed as described in the “Methods” section. Magnified insets highlight the morphology of activated glial cells in lumbar spinal cords (a) or in thoracic spinal cords (c). Infiltrating leukocytes (b, d; upper panel) and demyelination (b, d; lower panel) were detected by H&E staining and LFB staining, respectively, in lumbar (b) and thoracic (d) spinal cords. Arrows indicate infiltrating leukocytes (b, d; upper panel) and demyelination (b, d; lower panel). Data are representative of three independent experiments