Fig. 1

B cell counts and antibody titers are markedly reduced in the serum and CSF of JhD/MRL/lpr and hCD20-DTA/MRL/lpr mice. FACS analysis of CD19 positive cells in the peripheral blood of JhD/MRL/lpr and hCD20-DTA/MRL/lpr mice is shown in (a). The % CD19+ cells is provided in the red box in each panel. The MRL/lpr strain is used as a positive control. Total IgG levels (b) and anti-dsDNA IgG titers (c) in the serum and CSF of JhD/MRL/lpr and hCD20/MRL/lpr were measured by ELISA. Anti-NMDA receptor antibodies in the serum of JhD/MRL/lpr and hCD20-DTA/MRL/lpr mice are shown in (d). (JhD and control mice at 12–15 weeks of age: LPR mice, n = 5–8; JhD mice, n = 5–8; MPJ mice, n = 5–8; hCD20-DTA and control mice at 15 weeks of age: LPR mice, n = 7; hCD20-DTA mice, n = 7; MPJ mice, n = 7; except for the CSF ELISA experiments, where the number of mice in the LPR, hCD20-DTA ,and MPJ group were 7, 4, and 4, respectively). The CSF ELISA was done once; all other experiments were repeated twice, with similar results