Fig. 3

Effects of OT pre-treatment on LPS-induced microglial activation. a BV-2 cells and primary microglia were pre-treated with or without OT (1 μM) for 2 h followed by LPS (500 ng/ml) for 24 h, then stained with anti-Iba-1 (red), and counterstained with DAPI (blue). Quantification of the Iba-1 in BV-2 (b) and primary microglia (c) was determined by Image-Pro Plus 6.0. Scale bar = 50 μm. Images are representative of triplicate sets. Values were expressed relative to the fluorescence signal of respective controls. Values represent the mean ± S.D. **p < 0.01, ***p < 0.001 LPS VS Con; #p < 0.05, ##p < 0.01 LPS+ OT VS LPS