Fig. 5

Effects of OT on LPS-induced COX-2 and iNOS mRNA and protein expression in microglia. a BV-2 cells were pre-treated with or without OT (0.1–10 μM) for 2 h followed by LPS (500 ng/ml) for 24 h. The levels of COX-2 and iNOS were analyzed by Western blotting, and β-actin was used to evaluate protein loading. b BV-2 cells were pre-treated with or without OT (0.1–10 μM) for 2 h followed by LPS (500 ng/ml) for 4 h. The relative expression levels of COX-2 and iNOS gene were analyzed by RT-PCR. c Primary microglia were pre-treated with or without OT (1 μM) for 2 h followed by LPS (500 ng/ml) for 24 h. The levels of COX-2 and iNOS were analyzed by Western blotting, and β-actin was used to evaluate protein loading. d Primary microglia were pre-treated with or without OT (1 μM) for 2 h followed by LPS (500 ng/ml) for 24 h. The levels of COX-2 and iNOS mRNA were analyzed by RT-PCR, and β-actin was used to evaluate protein loading. Each value was normalized to β-actin. Values represent the mean ± S.D. of three independent experiments. ***p < 0.001 LPS VS Con; #p < 0.05, ##p < 0.01, LPS+ OT VS LPS