Fig. 5

Analysis of IgG autoantibody depositions. a Autoantibody depositions were analyzed as immunoglobulin G (IgG)-positive depositions per mm retina in naso-temporal cross-sections of the eye. A significant increase of IgG autoantibody depositions could be observed in fellow and injured eyes of the iOHT group (p < 0.05 and p < 0.01, respectively) compared with control eyes, but not in iOHT + Belimumab group. Significant values are indicated as follows: *p < 0.05, **p < 0.01. b–f Representative images of retinal cross-sections (×20 magnification) show distinct IgG-positive depositions (arrows) in the ganglion cell layer of the retina. Magnifications highlight the dense morphology of these depositions (b, d, and f). Triangles indicate IgG-positive blood vessels containing erythrocytes, which must not be confused with IgG depositions. Abbreviations: GCL ganglion cell layer, INL inner nuclear layer, ONL outer nuclear layer, CHO choroid. Scale bar represents 100 μm. Significant values are indicated as follows: *p < 0.05, **p < 0.01