Fig. 2

6-MP does not inhibit LPS-induced activation of MAPKs signals. BV-2 cells (a) and primary microglia (b) were pretreated with vehicle or 6-MP (50 μM) for 16 h and stimulated with LPS (100 ng/ml) for the indicated times. Whole cell extracts were prepared. Western analysis was used to determine LPS-induced p38, ERK, and JNK activation in the absence or presence of 6-MP. The immunoblots are representative of three independent experiments