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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: 6-Mercaptopurine attenuates tumor necrosis factor-α production in microglia through Nur77-mediated transrepression and PI3K/Akt/mTOR signaling-mediated translational regulation

Fig. 5

6-MP reduces LPS-induced p65 and coactivator p300 recruitment and histone H3 acetylation at the TNF-α promoter. a–c BV-2 cells were stimulated with 100 ng/ml LPS for the indicated times and chromatin immunoprecipitation (ChIP) assays were performed as described in the “Methods” section. The TNF-α promoter regions encompassing κB1-κB4 were targeted for ChIP analyses assessing associations of p65 (a), p300 (b), and acetylated histone H3 (Ac-H3) (c). The abundance of the immunoprecipitated DNA in a sample was normalized to the amount of signal in the input DNA. The values of the untreated samples were set to 1.0. Data are presented as mean ± SEM for three independent experiments. d–f BV-2 cells were pretreated with vehicle or 6-MP (50 μM) for 16 h followed by exposure to 100 ng/ml LPS for 60 min. ChIP analyses were carried out with anti-p65 (d), anti-p300 (e), and anti-Ac-H3 (f) antibodies. The values of the LPS alone-treated samples were set to 100 %. Data are presented as mean ± SEM for five independent experiments. *p < 0.05; **p < 0.01 compared with respective LPS alone

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