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Fig. 7 | Journal of Neuroinflammation

Fig. 7

From: 6-Mercaptopurine attenuates tumor necrosis factor-α production in microglia through Nur77-mediated transrepression and PI3K/Akt/mTOR signaling-mediated translational regulation

Fig. 7

Nur77 is required for 6-MP-mediated inhibition of p65 post-translational modifications, p65 recruitment, and histone H3 acetylation. BV-2 cells were transfected with control or Nur77 siRNA for 48 h. Cells were pretreated with 50 μM 6-MP for 16 h followed by stimulation with 100 ng/ml LPS for 20 min (a) or 60 min (b–e). The levels of phosphorylated p65 (Ser276) (a) and acetylated p65 (Lys310) (b) were determined by western blotting. ChIP assays were performed with anti-p65 (c), anti-p300 (d), and anti-Ac-H3 (e) antibodies. The detection of the immunoprecipitated TNF-α promoter was analyzed by PCR with promoter-specific primers. *p < 0.05; **p < 0.01 compared with 6-MP-pretreated control siRNA transfected cells

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