Fig. 8

6-MP blocks PI3K/Akt/mTOR signaling. BV-2 cells were pretreated with vehicle or 6-MP (50 μM) for 4 h and then stimulated with LPS (100 ng/ml) for the indicated times. Western analysis was used to determine total and phosphorylated Akt, S6K and 4E-BP1 proteins in whole cell extracts. Data are presented as mean ± SEM for three independent experiments. *p < 0.05; **p < 0.01 compared with respective LPS alone