Fig. 9

Dose-dependently inhibitory effect of 6-MP on activation of PI3K/Akt/mTOR signaling cascades. BV-2 cells (a) or primary microglia (b) were pretreated with vehicle or various concentrations (a) or 50 μM (b) of 6-MP for 4 h followed by exposure to LPS (100 ng/ml) for 60 min. Western analysis was performed as described in Fig. 8. Data are presented as mean ± SEM of three independent experiments. *p < 0.05; **p < 0.01 compared with LPS alone