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Fig. 3 | Journal of Neuroinflammation

Fig. 3

From: A novel antagonist of p75NTR reduces peripheral expansion and CNS trafficking of pro-inflammatory monocytes and spares function after traumatic brain injury

Fig. 3

EVT901 treatment reduced the number of Ly6Cint-high inflammatory monocytes in the circulation. Blood was obtained from C57Bl/6 WT mice at 1 and 6 weeks after TBI with or without EVT901. Leukocytes were isolated, stained with CD11b Ab and Ly6C Ab, and run on flow cytometry. TBI significantly increased the absolute number of CD11b + Ly6Cint-high monocytes in the circulations, while EVT901 treatment reduced the number. Two-way ANOVA revealed a significant effect of condition (F 3,25 = 15.85, p < 0.000006, η 2 = 0.67, power = 1.0), time (F 1,32 = 5.71, p = 0.025, η 2 = 0.19, power = 0.63), time × treatment (F 3,32 = 9.46, p < 0.0002, η 2 = 0.54, power = 0.99). Tukey’s post hoc tests showed there was a significant difference between TBI-vehicle and all other conditions, indicating that TBI increases monocyte numbers at 1 week but not 6 weeks and that EVT901 reduced this effect (*p < 0.05, ***p < 0.001). (n = 3 for sham and n = 4–7 for TBI)

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