Fig. 4

EVT901 treatment reduced the recruitment of Ly6C^int-high monocytes into the injured brain at 1 and 6 weeks after TBI. The injured hemisphere was dissected and homogenized. Single cells were obtained using percoll gradients and then stained with Abs against CD45, CD11b+, and Ly6C. a–c TBI significantly increased the absolute number of Ly6C^int-high inflammatory monocytes in the injured brain, while EVT901 treatment reduced the number. Two-way ANOVA revealed significant main effects of treatment (F _3,36 = 6.19, p = 0.002, η ² = 0.4, power = 0.94) and time (F _1,3 = 5.10, p < 0.032, η ² = 0.15, power = 0.59). Tukey’s post hoc tests showed that TBI significantly increased CD45^highCD11b + Ly6C^int-high inflammatory monocytes in the injured brain at both 1 and 6 weeks after TBI, while EVT901 treatment reduced the number in the injured brain, *p < 0.05. (n = 3–4 for sham and n = 5–7 for TBI)