Fig. 3

CD47 IR in control, FCD IIb, and TSC specimens. a, b CD47 IR in control specimens. a Strong somatic staining of CD47 in neurons (arrows and inset) within cortex. b Sporadic CD47 IR in glial cells (arrowheads) within white matter and co-localization of CD47 (green) and HLA-DR (red) in a microglia (inset). c, d CD47 IR in cortical lesions of FCD IIb. c Weak CD47 IR in dysmorphic neurons (arrows). d Negative balloon cells (arrowheads and inset) and weak CD47 IR in balloon cells (arrows). e–g CD47 IR in cortical tubers of TSC. e Negative dysmorphic neurons (arrowheads) and weak to moderate staining in dysmorphic neurons (arrows). f Weak CD47 IR in giant cells (arrows) and in a glial cell (arrowhead). g High magnification showing a giant cell with weak CD47 IR. h–j Double labeling in cortical lesions of FCDIIb specimens. h Co-localization of CD47 (green) with NF (red) in dysmorphic neurons. i Absence of co-localization between CD47 (green) and GFAP (red) in astrocytes (arrows: astrocytes, arrowhead: dysmorphic neuron). j Co-localization of CD47 (green) and HLA-DR (red) in a microglia (arrow; arrowhead: dysmorphic neuron). k–m Double labeling in cortical tubers of TSC specimens. k Co-localization of CD47 (green) with NF (red) in giant cells. l Absence of co-localization between CD47 (green) and GFAP (red) in astrocytes (arrows: astrocytes, arrowhead: giant cell). m Co-localization of CD47 (green) and HLA-DR (red) in microglia (arrows; arrowhead: giant cell). Scale bars: a 40 μm; b 35 μm; d, e: 50 μm; f 30 μm, g 20 μm; h–m 50 μm