Fig. 10

Induction of IFN-I and ISGs in primary cortical neurons derived from IDO KO mice after JEV infection. Primary cortical neurons generated from BL/6 and IDO KO mice were infected at MOIs of 0.001 and 0.01, and viral replication and IFN-I responses 24, 48, and 72 h pi were analyzed by real-time qRT-PCR. a JEV replication. b IFN-I (IFN-α/β) expression. c–e Induction of ISGs, RLRs, and IRFs in infected primary cortical neuron. The expression of IFN-I (b), ISGs (c), RLRs (d), and IRFs (e) was determined using primary cortical neuron infected with JEV (0.1 MOI) 24 h pi. The bar charts show the average ± SD of values derived from primary cortical neurons in quadruplicate. *p < 0.05; **p < 0.01; ***p < 0.001 compared with levels in the indicated groups