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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: Blockage of indoleamine 2,3-dioxygenase regulates Japanese encephalitis via enhancement of type I/II IFN innate and adaptive T-cell responses

Fig. 5

IDO ablation enhances CD4+ and CD8+ T-cell responses specific for JEV antigen. a JEV E-specific IgM and IgG response. Sera were collected from surviving mice 7 dpi and used in ELISA to detect IgM and IgG levels specific for the JEV E protein. The data show the average ± SD of the JEV E-specific IgM and IgG levels derived from surviving mice (n = 6–8). b, c CD4+ T-cell response specific for JEV antigen. The splenocytes prepared from surviving mice (n = 4–5) were stimulated with the JEV epitope peptides of CD4+ T-cells (NS1132–145 and NS3563–574) for 12 h. The frequency (b) and absolute number (c) of CD4+ T-cells specific for the JEV epitope peptides were determined by intracellular CD154, IFN-γ, or TNF-α staining, combined with surface CD4 staining. d, e CD8+ T-cell response specific for JEV antigen. The frequency (d) and absolute number (e) of CD8+ T-cells specific for the JEV epitope peptide (NS4B215–223) were determined by intracellular IFN-γ or TNF-α staining after an 8-h stimulation with peptide. Values in the representative dot plots denote the average percentage of the indicated cell population, and the bar charts show the average ± SD of the values derived from at least four mice per group. *p < 0.05; **p < 0.01; ***p < 0.001 compared with levels in the indicated groups

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