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Fig. 6 | Journal of Neuroinflammation

Fig. 6

From: Blockage of indoleamine 2,3-dioxygenase regulates Japanese encephalitis via enhancement of type I/II IFN innate and adaptive T-cell responses

Fig. 6

IDO induces a skewed IFN-γ+CD4+ T-cell response during JE. a, b The frequency and number of CD4+Foxp3+ Tregs in the spleen of IDO-ablated mice. The frequency (a) and absolute number (b) of CD4+Foxp3+ Treg cells in the spleen of wild-type and IDO KO mice were determined by flow cytometric analysis 5 dpi. The values in the representative dot plots show the average percentage of CD25+Foxp3+ cells in CD4+ T-cells; the bar charts denote the average number ± SD of CD4+Foxp3+ Tregs in the spleen derived from at least four mice per group. c, d The frequency and number of IFN-γ+CD4+ Th1 and IL-17+CD4+ Th17 cells in the spleen of IDO-ablated mice. The frequency and number of IFN-γ+CD4+ Th1 and IL-17+CD4+ Th17 cells were determined by intracellular cytokine staining of the splenocytes prepared from wild-type and IDO KO mice 5 dpi in response to PMA + ionomycin stimulation. The values in the representative dot plots show the average percentage of the indicated cell populations after gating on CD4+ T-cells; the bar charts denote the average number ± SD of IFN-γ+CD4+ Th1 and IL-17+CD4+ Th17 cells in the spleen derived from at least four mice per group. **p < 0.01 compared with levels in the indicated groups

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