Fig. 4

Proliferation of fibroblasts and astrocytes is not altered in APRIL KO mice after SCI. EdU⁺ cells (white) are widely distributed throughout the GFAP (red) and PDGFRβ (green) regions in APRIL KO (j) and WT (a–i) animals. High-magnification images of proliferating fibroblasts (b–e) and astrocytes (f–i) are shown. Quantification of proliferating fibroblasts (k) and astrocytes (l) reveals no difference between APRIL KO and WT mice following SCI. b–e From top dashed square region in (a). f–i From bottom dashed region in (a). Percent in k and l are calculated from the total number of PDGFRβ⁺ or GFAP⁺ cells within the quantified region. n = 3 per group (biological replicates). Scale bar = 250 μm (a, j), 10 μm (b–i)