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Fig. 2 | Journal of Neuroinflammation

Fig. 2

From: Methamphetamine alters microglial immune function through P2X7R signaling

Fig. 2

METH increases migratory capacity of microglia that is P2X7R-dependent. In vitro migration was performed using the transwell migration assay. ESdM cells (5 × 104) were loaded with 5 mM Calcein-AM. In the lower chamber of the transwell plate, 10 ng/mL of fractalkine (CX3CL1) was added to determine the number of migrating cells and was visualized under ×40 magnification. a shows representative images of control (a), 10 μM P2X7R-antagonist A 438079 (b), 100 μM METH (c), and 100 μM METH and 10 μM P2X7R-antagonist A 438079 (d) treated ESdM cells. b Quantification of total cells migrated in response to 100 μM METH and/or A 438079 represents a statistically significant increase in migration towards CX3CL1 in METH-treated cells as compared to control (****p < 0.0001) that is reversed by pretreatment with A 438079 (****p < 0.0001) (ANOVA)

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