Fig. 1

Assessment of AQP11 expression with immunohistochemistry, scale bars = 20 μm. a Expression in equine retina. AQP11 (red) was specifically expressed at retinal Müller glial cells, reaching from endfeet in ganglion cell layer to outer limiting membrane; ILM inner limiting membrane (arrow), GCL ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, OPL outer plexiform layer, ONL outer nuclear layer, OLM outer limiting membrane (arrowhead). b Negative control, incubated only with secondary antibody anti mouse IgG Alexa 568. c Overlay of Müller glia marker vimentin (green) and AQP11 expression (red) in equine retina results in yellow color. d Müller glia cell membrane localization of AQP11 (violet) stained with fine-grained resolution. e Preincubation of AQP11 antibody with 100 μg/ml irrelevant peptide did not interfere with antibody binding, AQP11: red. f Preincubation with 100 μg/ml AQP11 immunization peptide completely blocked antibody binding. g Preincubation with 1 μg/ml AQP11 immunization peptide partly blocked antibody binding. h Müller glia endfeet of mouse and i rat retina express AQP11 (red). j AQP11 (red) expression in positive control tissue mouse kidney