Fig. 4

AQP11 plays a role in cell volume regulation of Müller glial cells. Hyperglycemic (a), hypotonic (b), and hypertonic (c) extracellular environments led to significant respective cell volume regulations. Inhibition of AQP4 (blue bar), AQP5 (green bar), or AQP11 (red bar) did not change cellular response under hyperglycemic conditions (Fig. 4a). Under hypotonia, only blockage of AQP5 rescued the cells from swelling (Fig. 4b, green bar). In contrast, inhibition of AQP4 and AQP11 channels did not change reaction in hyperglycemia and hypotonia (Fig. 4a, b) but significantly prevented reduction of cell size in hypertonic condition (Fig. 4c, blue bar AQP4, red bar AQP11). **p ≤ 0.01; ***p ≤ 0.001