Fig. 2

Upregulation of AIF1 is attenuated in Bax-deficient mice after optic nerve crush. Cross sections of eye cups were analyzed for changes in AIF1 expression after crush injury. a–d Although a baseline level of AIF1-positive cells (red label) populated the retina in control eyes, following optic nerve crush Bax ^+/+ retinas exhibited an increase in the number of AIF1-positive cells. Microglial cells appeared in the inner plexiform layer (IPL), outer plexiform layer (IPL), and ganglion cell layer (GCL). e–h In Bax ^−/− mice, the amount of AIF1 labeling was significantly attenuated after crush, and the intensity of AIF1 labeling did not appear to change. Inner nuclear layer (INL), outer nuclear layer (ONL). DAPI nuclear counterstain (blue label). Scale bar 50 μm. For each genotype at each time point, n ≥ 3