Fig. 5

Microglial activation is present but reduced in the optic nerve head of Bax ^−/− mice after crush. The microglial populations in the optic nerve heads of Bax ^+/+ and Bax ^−/− mice were also compared. a–c Wild-type mice exhibited a clear increase in the intensity and number of AIF1-expressing cells (red label) at both 7 and 14 days after crush. d–f Unlike in the retina, mice deficient for Bax also exhibited an increase in microglia at the optic nerve head 7 and 14 days post-crush, although the number of cells and intensity did not appear as high as the wild-type counterparts. DAPI nuclear counterstain (blue label). Scale bar 50 μm. For each genotype at each time point, n ≥ 3