Fig. 2

Aldara treatment induces a transcriptional brain response that is independent of a systemic peripheral response. Mice were treated with 80 mg Aldara cream or control cream every 24 h for one, three or five consecutive days. Mice were euthanised 24 h after the final application. Cardiac puncture was performed to retrieve PBLs, and perfused brains were extracted. RNA was isolated from both tissues, and a QRT-PCR analysis of the target chemokine genes was performed. b Luminex analysis was performed to determine the expression of classic inflammatory cytokines in the plasma. n = 4 mice per group. Significance was measured using two-way ANOVA with Bonferroni multiple comparison post-tests. Additional two-way ANOVA were used to compare the control and treated groups within each tissue (a). ***p ≤ 0.001 **p ≤ 0.01 *p ≤ 0.05