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Fig. 5 | Journal of Neuroinflammation

Fig. 5

From: TLR7-mediated skin inflammation remotely triggers chemokine expression and leukocyte accumulation in the brain

Fig. 5

Aldara treatment induces the infiltration of a number of lymphocyte subsets into the brain. Mice were treated with 80-mg Aldara cream or control cream every 24 h for one, three or five consecutive days. Mice were euthanised 24 h following the final application. a Protein expression of CXCL10 in whole brain lysate was assessed in control and treated brains 4 h, 12 h, 1 day, 3 days and 5 days after treatment. b Perfused brains were homogenised, and a single cell suspension was generated as described. Cells were labelled with fluorescent antibodies. CD11b cells were selected following dead cell exclusion, and doublet exclusion and representative gating strategy based on expression of NK1.1, TCRb, CD4, CD8, CD44 and CD62L is shown. c Counts per million cells were generated using total cell counts and are shown for NK cells, NK T cells, CD8lo T cells, CD8+ T cells and CD4+ T cells at 1, 3 and 5 days following Aldara treatment. n = 4 mice per group. Significance was determined using one- (a) or two-way (b, c) ANOVA with Bonferroni multiple comparison post-tests. Data shown in a were log transformed prior to statistical analysis ****p < 0.0001 ***p ≤ 0.001 **p ≤ 0.01

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