Fig. 5

Aldara treatment induces the infiltration of a number of lymphocyte subsets into the brain. Mice were treated with 80-mg Aldara cream or control cream every 24 h for one, three or five consecutive days. Mice were euthanised 24 h following the final application. a Protein expression of CXCL10 in whole brain lysate was assessed in control and treated brains 4 h, 12 h, 1 day, 3 days and 5 days after treatment. b Perfused brains were homogenised, and a single cell suspension was generated as described. Cells were labelled with fluorescent antibodies. CD11b⁻ cells were selected following dead cell exclusion, and doublet exclusion and representative gating strategy based on expression of NK1.1, TCRb, CD4, CD8, CD44 and CD62L is shown. c Counts per million cells were generated using total cell counts and are shown for NK cells, NK T cells, CD8^lo T cells, CD8⁺ T cells and CD4⁺ T cells at 1, 3 and 5 days following Aldara treatment. n = 4 mice per group. Significance was determined using one- (a) or two-way (b, c) ANOVA with Bonferroni multiple comparison post-tests. Data shown in a were log transformed prior to statistical analysis ****p < 0.0001 ***p ≤ 0.001 **p ≤ 0.01