Fig. 2

Ido1, Ido2, and Tdo2 transcript expression differ between tissues and across brain regions. Top: Two brain regions (frontal cortex, FrCor; hippocampus, Hip), the lung and liver, were collected from naïve mice (n = 6). Expression of two transcripts for each DO is presented to illustrate tissue and brain region specificity. The sample type with the lowest expression was set to 1.0, with other samples showing relative expression levels within transcript (i.e., across row, C _t values for FrCor are shown to illustrate relative amplification across transcripts). The tissue with the highest relative expression for each transcript is highlighted for emphasis. Total DO expression (Ido1-Tot, Ido2-Tot, and Tdo2-Tot) was assessed using assays that span exons conserved across all transcripts within each gene. Bottom: Similarly, brain regions were collected to quantify differences in expression levels across additional brain regions of naïve mice (n = 4). Expression within the frontal cortex was set to 1.0 for all transcripts, and other regions show expression relative to the FrCor. C _t values for each transcript (a: Ido1-FL, b: Ido1-v1, c: Ido1-Tot, d: Ido2-FL, e: Ido2-v1, f: Ido2-Tot, g: Tdo2-FL/v1, h: Tdo2-v1, I: Tdo2-Tot) within the FrCor are shown to indicate relative amplification level during the qPCR reaction (Ido1-FL is essentially absent in the naïve mouse brain; samples without a calculated C _t value are assigned a value of 40 for presentation). For both data sets, mice (10- to 12-week old) were euthanized under CO₂ and then perfused with cold saline to minimize blood content within samples prior to tissue or brain region excision. Samples were then processed for qPCR analysis as described in the “Methods” section for OHSC samples