Fig. 4From: Decreased neuroinflammation correlates to higher vagus nerve activity fluctuations in near-term ovine fetuses: a case for the afferent cholinergic anti-inflammatory pathway?α7nAChR + HMGB1 + Iba1 + DAPI immunofluorescent staining: Red = Iba1; Blue = DAPI; Green = HMGB1; White = α7nAChR. Images were made from extended depth of focus, maximum intensity projections from convexial gray matter layers 4 to 6. Top: Features of active versus inactive microglia: Inactive cells have no interaction with neurons, are smaller, less bright, and less ramified versus the activated cells. Bottom: Co-localization staining for Iba1+ cells expressing α7nAChR with intracellular localization of HMGB1 and DAPI counterstain. a Iba1 + DAPI + α7nAChR; b Iba1 + DAPI + HMGB1; c Iba1+ α7nAChR; d best focus color composite of Iba1+ α7nAChR + HMGB1 + DAPIBack to article page