Fig. 5
From: Induction of the type I interferon response in neurological forms of Gaucher disease
Induction of the IFN response in neurons and downstream signaling in microglia. a Elevation of IFNβ in neurons in the cerebral cortex of 18-day-old CBE-treated mice (100 mg/kg, from 8 days of age) compared to untreated mice (control). Double immunofluorescence using either anti-GFP (IFNβ, green) and anti-NeuN (red), anti-GFP and anti-MAC2 (red), or anti-GFP and anti-GFAP (red) antibodies and the nuclear marker, DAPI (blue). Scale bar, 10 μm. Results are representative of two experiments. b qPCR analysis of various genes in cortical homogenates from 18-day-old Ifnar flox/flox; CX3CR1-Cre + (n = 4) and Ifnar flox/flox; CX3CR1-Cre- (n = 4) mice treated with CBE (100 mg/kg per day) from 8 days of age for 10 days. Results are expressed as fold change of CBE-treated versus control (n = 3–4) mice and are means ± s.e.m. CT values were normalized to levels of HPRT. *p < 0.05, **p < 0.01, ***p < 0.001