Fig. 4

MV infection does not alter BrdU uptake by NSPCs. CD46+ (a-c) and CD46+/IFNγ-KO (d-f) pups were injected with BrdU 24 h before whole-brain isolates were processed and labeled for BrdU+ cells (a, d), or BrdU(+)Nestin(+) (b, e), and BrdU(+)CD24(+) (c, f) double-positive cells. Reduced BrdU uptake was observed in MV-infected CD46+/IFNγ-KO pups at 3 and 7 dpi (d), but not CD46+ mice (a). BrdU+ cells within the Nestin+ population showed no significant difference between control and MV-infected pups in both genotypes, but a significant reduction with age (b, e). The percentage of BrdU+ cells within the CD24+ cell population was reduced at 10 dpi (c, f), when severe illness and death occurs. *p < 0.01 significantly different uninfected versus MV-infected; #p < 0.05 significantly different between dpi