Fig. 6From: Interferon gamma protects neonatal neural stem/progenitor cells during measles virus infection of the brainImpact of MV infection on expression of NSPC, neuronal, and glial markers in the hippocampus. Whole tissue lysates of hippocampal explants from CD46+ (a–d) and CD46+/IFNγ-KO (e–h) control and MV-infected mice were analyzed by Western Blots for Nestin (NSPC; a–e), DCX (early neuron; b, f), βIII-tubulin (mature neuron; c, g) and GFAP (astrocyte; d, h) proteins. Integrated intensity values for each band, which are proportional to the sum of the fluorescent signal (measured in pixels) enclosed by the region of interest, are normalized to GAPDH as a protein loading control. Changes in nestin and DCX hippocampal protein expression correspond with changes in cell number observed for whole-brain cell isolates. *p < 0.01 significantly different uninfected versus MV-infected; #p < 0.05 significantly different between dpiBack to article page